Simvastatin이 UMR-106 세포의 조골세포 형성에 미치는 영향
Effects of Simvastatin on osteogenesis of rat osteoblast-like cells, UMP-106
황의관, 류동목, 지유진, 이덕원, 이현우,
소속 상세정보
황의관 ( Hwang Eui-Kwan ) - 경희대학교 일반대학원 치의학과 구강악안면외과학
류동목 ( Ryu Dong-Mok ) - 경희대학교 치의학전문대학원 구강악안면외과학교실
지유진 ( Jee Yu-Jin ) - 경희대학교 치의학전문대학원 구강악안면외과학교실
이덕원 ( Lee Deok-Won ) - 경희대학교 일반대학원 치의학과 구강악안면외과학
이현우 ( Lee Hyeon-Woo ) - 경희대학교 치의학전문대학원 치과약리학교실
KMID : 0362720080460090563
Abstract
Purpose : The purpose of this study is to investigate the effects of Simvastain, which is HMG-CoA reductase inhibitor, on proliferation and differentiation of osteoblast.
Materials & Methods : Twenty-four cell culture plates containing essential medium were seeded with UMR-106 cell lines, at density of 5 x cells per plate. Each plates were incubated with 5% incubator . Starting from 2 days after incubation, cell culture medias were replaced with Osteogenesis induction media every 2 days, for 12 days. In some plates, 0.01, 0.1, 1, 10, of Simvastatin were added with Osteogenesis induction media, and classified as "test group". Those not added with Simvastatin were classified as "control group".
Results : 1. When Alrizarin Red staining was observed with naked eye, control group showed normal deep red color, but test group show rapid decrease of red color as Simvastatin concentration increased more than . 2, When observed with microscope, compared to control group, amount of osteo matrix stained with Alrizarin Red decreased rapidly in Simvastatin concentration more than . 3. In optical density analysis, regarding control group as a basis, mineral deposition decreased rapidly when Simvastatin concentration increased more than . 4. In flow cytometry analysis, survival rate of UMR-106 cell showed no changes in both control group and test group.
Conclusion : From the above results, we were able to identify that Simvastatin inhibited osteogenesis without effecting survival or cell number of osteoblasts.
키워드
Osteogenesis;Simvastatin;UMR-106
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